USPTO Patents Application 10561839

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= (54) Title: MICROWELL ARRAY CHIP AND ITS MANUFACTURING METHOD
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(57) Abstract: A microwell array chip comprising microwclls in one
major surface of a substrate. Each microwell has such a shape and di-
mensions that it contains only one living cell. On the major surface of
the substrate where the openings of the microwells are provided, mark-
ers for the microwells are provided. Another microwell array chip com-
prising microwells in one major surface of a substrate. Each microwell
has such a shape and dimensions that it contains only one living cell.
A projection portion narrowing the opening of each microwell is dis-
posed in the opening. A method for manufacturing the microwell array
chip comprises a step of forming a film at least on one major surface
of a substrate, a step of applying a resist to the formed film, a step of
exposing the resist surface through a mask having a microwell pattern
and removing the uncured portion of the resist, a step of making a hole
of microwell array shape by etching the film and the exposed portion
of the substrate, and a step of removing the resist Further, another mi-
crowell array chip of a silicon having microwells in each of which one
living cell of a subject is contained. Each microwell has such a shape
and dimensions that it contains only one living cell.

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INTERNATIONAL SEARCH REPORT

A. CLASSIFICATION OF SUBJECT MATTER

Int. CI 7 G01N33/53, G01N37/00

International application No.

PCT/JP2004/014285

According to International Patent Classification (IPC) or to both national classification and IPC
B. FIELDS SEARCHED

Minimum documentation searched (classification system followed by classification symbols)
Int. CI 7 G01N33/53, G01N37/00

Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched
Jitsuyo Shinan Koho 1922-1996 Toroku Jitsuyo Shinan Koho 1994-2005

Kokai Jitsuyo Shinan Koho 1971-2005 Jitsuyo Shinan Toroku Koho 1996-2005

Electronic data base consulted during the international search (name of data base and, where practicable, search terms used)

C. DOCUMENTS CONSIDERED TO BE RELEVANT

Category*

Citation of document, with indication, where appropriate, of the relevant passages

Relevant to claim No.

Y
A

WO 2002/055653 A (Matsushita Electric
Industrial Co. , Ltd. ) ,
18 July, 2002 (18.07.02),

& US" 2003-0113833 A & EP 1352952 A
& CN 1458972 A

1-12,23-32
13-22

Y
A

JP 5-240869 A (Suzuki Motor Corp.),
21 September, 1993 (21.09.93),
(Family: none)

1-12,23-32
13-22

Y
A

JP 63-106565 A (E.I. Du Pont De Nemours & Co.),
11 May, 1988 (11.05.88) ,
& EP 258565 A

1-12,23-32
13-22

Y
A

JP 2003-33177 A (Mitsuo OKANO) ,
04 February, 2003 (04.02.03),
(Family: none)

1-12,23-32
13-22

a Further documents are listed in the continuation of Box C.

□ See patent family annex.

• Special categories of ci ted documents:

"A" document defining the general state of the art which is not considered
to be of particular relevance

"E" earlier application or patent but published on or after the international
filing date

"L" document which may throw doubts on priority ctaim(s) or which is
cited to establish the publication date of another citation or other
special reason (as specified)

"O" document referring to an oral disclosure, use, exhibition or other means
"P" document published prior to the international filing date but later than
the priority date claimed

"V later document published after the international filing date or priority
date and not in conflict with the application but cited to understand
the principle or theory underlying the invention

"X* document of particular relevance; the claimed invention cannot be
considered novel or cannot be considered to involve an inventive
step when the document is taken alone

■ Y" document of particular relevance; the claimed invention cannot be
considered to involve an inventive step when the document is
combined with one or more other such documents, such combination
being obvious to a person skilled in the art
document member of the same patent family

Date of the actual completion of the international search
15 March, 2005 (15.03.05)

Date of mailing of the international search report
29 March, 2005 (29.03.05)

Name and mailing address of the ISA/

Japanese Patent Office

Authorized officer
Telephone No.

Form PCT/IS A/2 10 (second sheet) (January 2004)

mmmm^ pct/jp2oo4/o 14285

a. &m<om-t%ftm<o$rm. m&¥ftf&m (ipo )

Int. C 1 7 GO 1 N3 3/5 3, G01N37/00

»HE*:*?ofc«/NRjm (BRttl&ttR (IPC) )

Int. CI 7 G01N3 3/53, G01N37/00

B*H3lffiff^^fl 1 922-1 9 9 6^

B#B2rRl2IJB?&rS&fc« 1 9 7 1-200 5¥

&*mamMfaM&!&i 1994-2005^

B*m£:M%TM&M2t¥i 1996-2005^

c.

BarfsfcWft fetus Aft

HM-S

Y
A

Y
A

WO 2 0 0 2/0 5 5 6 5 3
0 2.0 7.1 8

& US 2003—0113833
& EP 1352952 A
& CN 1458972 A

A (feTS^l
A

2 0

JP 5-240869 A (;* X^ttSfc^fcfc) 1 9 9 3. 0 9
2 1

1-12,
2 3 — 32
13 — 22

1 — 12,

2 3-32
13-2 2

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TEj BRttJKBtfr<PtfUH£fcll#VI : -e*>sas % BRUMS

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Tyj ^t-BBjgwfcs^T-fcoT, ^zSCiSfcitfiioi^

•fcoT^t£riS&V>fc#;i fetus to
r&j p-/N'fyh77;y-M

1 5. 03. 2005

mmm*mm* 2Q _ 3 _ 2Q05

B^H^fF^r (I SA/J P)
»5{S!## 100-8915

WW*** (*K*>*5«ft>

03-3581-1101 F>

2 J

9 5 0 7

3 2 5 1

»CT/ISA/2 10 («2^) (2004^11)

B^tt}ffi#-*§- PCT/JP20 04/01 428 5

C , Oftfe)

JP 63-106565 A (-f— • T-f • T^/tfV
T — X • 7y K • =i 1988. 05. 11

& EP 258565 A

K •

JP 2003-33177 A
0 4

2 0 0 3. 0 2.

1-12,
2 3 — 32
13-2 2

1-12,
2 3-3 2
13-2 2

flKPCT/I SA/2 1 0 (&2-i— S?©i6fe#) (2004^1^)

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